Hotspot

trnN-GUU-rpl32

SSC · 110350-111016 · 667 bp

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Pi 0.022
MarkerSeek score 68.20
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
667
variable_sites
30
pi
0.022
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG TTTGACATAGAAGTACGTTTTT 50.0 27.3 58.1 52.0 745-890 bp 1.0 46.0
trnN-GUU-rpl32_p2 AGTCAGTTAACAGCCAACCG TTTTGACATAGAAGTACGTTTT 50.0 27.3 58.1 52.0 746-891 bp 1.0 46.0
trnN-GUU-rpl32_p3 AGTCAGTTAACAGCCAACCG TTTTTGACATAGAAGTACGTTT 50.0 27.3 58.1 52.0 747-892 bp 1.0 46.0
trnN-GUU-rpl32_p4 ACGACCAGTCAGTTAACAGC TTTGACATAGAAGTACGTTTTT 50.0 27.3 57.8 52.0 751-896 bp 1.0 45.9
trnN-GUU-rpl32_p5 ACGACCAGTCAGTTAACAGC TTTTGACATAGAAGTACGTTTT 50.0 27.3 57.8 52.0 752-897 bp 1.0 45.9