Hotspot

petN-psbM

LSC · 29949-30890 · 942 bp

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Pi 0.0351
MarkerSeek score 78.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
942
variable_sites
63
pi
0.0351
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GTATGGGGAAGGAGTGGACT CGTTTATTGCTACTGCGCTG 55.0 50.0 58.1 57.9 666-1151 bp 1.0 87.4
petN-psbM_p2 AGTATGGGGAAGGAGTGGAC CGTTTATTGCTACTGCGCTG 55.0 50.0 58.1 57.9 667-1152 bp 1.0 87.3
petN-psbM_p3 ATGGGGAAGGAGTGGACTTT CGTTTATTGCTACTGCGCTG 50.0 50.0 58.2 57.9 664-1149 bp 1.0 86.4
petN-psbM_p4 AGTATGGGGAAGGAGTGGAC ACGTCAAAACAGTCAGCCAA 55.0 45.0 58.1 58.3 610-1095 bp 1.0 86.2
petN-psbM_p5 GTATGGGGAAGGAGTGGACT ACGTCAAAACAGTCAGCCAA 55.0 45.0 58.1 58.3 609-1094 bp 1.0 86.2