Hotspot

trnL-TAG

SSC · 116726-116805 · 80 bp

Back to Luffa

Pi 0.569
MarkerSeek score 76.00
Primer available yes
Label trnL-TAG

Feature Metadata

label_name
trnL-TAG
feature_type
tRNA
region
SSC
length_bp
80
variable_sites
41
pi
0.569
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-TAG_p1 GGAAACGCTCAAATCCCCTA GGAGAGCGGGGACTAATAGT 50.0 55.0 57.6 58.0 752 bp 0.5 56.7
trnL-TAG_p2 GGAAACGCTCAAATCCCCTA TGGAGAGCGGGGACTAATAG 50.0 55.0 57.6 58.0 753 bp 0.5 56.7
trnL-TAG_p3 GGAAACGCTCAAATCCCCTA CGGGGACTAATAGTGGGGAT 50.0 55.0 57.6 58.0 746 bp 0.5 56.5
trnL-TAG_p4 GGAAACGCTCAAATCCCCTA AAAATAGTACTTGGCGCGGT 50.0 45.0 57.6 57.9 681 bp 0.5 55.9
trnL-TAG_p5 GGAAACGCTCAAATCCCCTA AAATAGTACTTGGCGCGGTT 50.0 45.0 57.6 57.9 680 bp 0.5 55.9