Hotspot

ndhI

SSC · 121690-122190 · 501 bp

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Pi 0.519
MarkerSeek score 77.90
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
SSC
length_bp
501
variable_sites
214
pi
0.519
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 AAACTGGGCGTTTGCTAGAA AAATAATCCCGACACGCTCC 45.0 50.0 58.0 58.1 1762 bp 0.5 59.4
ndhI_p2 CATGTATCGGTCCAGGCAAA AAATAATCCCGACACGCTCC 50.0 50.0 58.0 58.1 1616 bp 0.5 59.3
ndhI_p3 CAAGAACCAGTCCCAACGAA AAATAATCCCGACACGCTCC 50.0 50.0 58.0 58.1 1721 bp 0.5 59.3
ndhI_p4 CGGTCCAGGCAAATCCATTA AAATAATCCCGACACGCTCC 50.0 50.0 57.9 58.1 1609 bp 0.5 59.2
ndhI_p5 AAACTGGGCGTTTGCTAGAA TGGTTTTTGGGGGTGGAATT 45.0 45.0 58.0 57.8 1593 bp 0.5 58.2