Hotspot

ndhH

SSC · 124519-125700 · 1182 bp

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Pi 0.489
MarkerSeek score 82.00
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
517
pi
0.489
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 ATCCGTTGTTGTATCCCTGC CGGAAAACGCCAACGATTAC 50.0 50.0 58.0 58.1 1574 bp 0.5 58.7
ndhH_p2 ATCCGTTGTTGTATCCCTGC TGGCACTGTCTGACATTCTG 50.0 50.0 58.0 57.8 2094 bp 0.5 58.3
ndhH_p3 GATGGGAGCCACACTTGAAA CGGAAAACGCCAACGATTAC 50.0 50.0 58.1 58.1 1818 bp 0.5 58.3
ndhH_p4 TCAGGTCCAATCCGTTGTTG CGGAAAACGCCAACGATTAC 50.0 50.0 58.1 58.1 1583 bp 0.5 58.1
ndhH_p5 ATCCGTTGTTGTATCCCTGC TCGGAAAACGCCAACGATTA 50.0 45.0 58.0 58.2 1575 bp 0.5 58.0