Hotspot

ndhF

SSC · 112502-114748 · 2247 bp

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Pi 0.441
MarkerSeek score 80.30
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2247
variable_sites
876
pi
0.441
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACGAAGGGGACTAAACAGGA AGCGGGAAATTTTTGTAATGCA 50.0 36.4 58.0 57.7 2924 bp 0.5 41.7
ndhF_p2 ACAGGAACAAGAGGGATCCA AGCGGGAAATTTTTGTAATGCA 50.0 36.4 58.0 57.7 2910 bp 0.5 41.5
ndhF_p3 AAGAGGGATCCACCGAAGAA AGCGGGAAATTTTTGTAATGCA 50.0 36.4 58.0 57.7 2902 bp 0.5 41.5
ndhF_p4 CTCAGAAACGGAGGAAAGGG AGCGGGAAATTTTTGTAATGCA 55.0 36.4 57.9 57.7 2991 bp 0.5 41.1
ndhF_p5 GGGAAAGCGAGGAAGAAACA AGCGGGAAATTTTTGTAATGCA 50.0 36.4 58.1 57.7 2971 bp 0.5 41.0