Hotspot

ndhE

SSC · 120277-120579 · 303 bp

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Pi 0.482
MarkerSeek score 76.50
Primer available yes
Label ndhE

Feature Metadata

label_name
ndhE
feature_type
gene
region
SSC
length_bp
303
variable_sites
134
pi
0.482
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE_p1 ACCCATGCTTCGAGTTGTTT TTCGTTGGGACTGGTTCTTG 45.0 50.0 58.0 58.0 1438 bp 0.5 59.5
ndhE_p2 GCTTCGAGTTGTTTCATGCC TTCGTTGGGACTGGTTCTTG 50.0 50.0 58.0 58.0 1432 bp 0.5 59.4
ndhE_p3 ACACTCAAGAAATCCGTCGG TTCGTTGGGACTGGTTCTTG 50.0 50.0 57.9 58.0 1398 bp 0.5 59.1
ndhE_p4 CTTCTGTTCTTGGAGCCGAA TTCGTTGGGACTGGTTCTTG 50.0 50.0 57.8 58.0 1343 bp 0.5 58.3
ndhE_p5 TTCTGTTCTTGGAGCCGAAG TTCGTTGGGACTGGTTCTTG 50.0 50.0 57.8 58.0 1342 bp 0.5 58.3