Hotspot

ndhD

SSC · 118130-119539 · 1410 bp

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Pi 0.484
MarkerSeek score 78.20
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1410
variable_sites
515
pi
0.484
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.75
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 GCCTGACGAACACAAACATG AAACAACTCGAAGCATGGGT 50.0 45.0 58.0 58.0 1921 bp 0.5 59.6
ndhD_p2 GCCTGACGAACACAAACATG GGCATGAAACAACTCGAAGC 50.0 50.0 58.0 58.0 1927 bp 0.5 59.5
ndhD_p3 GCCTGACGAACACAAACATG CCGACGGATTTCTTGAGTGT 50.0 50.0 58.0 57.9 1961 bp 0.5 59.1
ndhD_p4 ATTCAAGAAAGGGCCTGACG AAACAACTCGAAGCATGGGT 50.0 45.0 58.2 58.0 1933 bp 0.5 58.5
ndhD_p5 ATTCAAGAAAGGGCCTGACG GGCATGAAACAACTCGAAGC 50.0 50.0 58.2 58.0 1939 bp 0.5 58.4