Hotspot

ndhI-ndhA

SSC · 122191-122270 · 80 bp

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Pi 0.507
MarkerSeek score 77.00
Primer available yes
Label ndhI-ndhA

Feature Metadata

label_name
ndhI-ndhA
feature_type
igs
region
SSC
length_bp
80
variable_sites
38
pi
0.507
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhA_p1 TGCAGCTCGTACTGTTTGTT AAATAATCCCGACACGCTCC 45.0 50.0 58.1 58.1 796 bp 0.5 59.2
ndhI-ndhA_p2 TTGCAGCTCGTACTGTTTGT AAATAATCCCGACACGCTCC 45.0 50.0 58.1 58.1 797 bp 0.5 59.2
ndhI-ndhA_p3 TTCCAATCAACAACGGGGAG AAATAATCCCGACACGCTCC 50.0 50.0 58.1 58.1 990 bp 0.5 58.8
ndhI-ndhA_p4 CCAATCAACAACGGGGAGAT AAATAATCCCGACACGCTCC 50.0 50.0 57.9 58.1 988 bp 0.5 58.6
ndhI-ndhA_p5 ACCAATGTACCTTGCAGCTC AAATAATCCCGACACGCTCC 50.0 50.0 58.2 58.1 808 bp 0.5 58.2