Hotspot

ndhG-ndhI

SSC · 121339-121689 · 351 bp

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Pi 0.423
MarkerSeek score 75.80
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
351
variable_sites
123
pi
0.423
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AAACTGGGCGTTTGCTAGAA AACAAACAGTACGAGCTGCA 45.0 45.0 58.0 58.1 986 bp 0.5 59.4
ndhG-ndhI_p2 AAACTGGGCGTTTGCTAGAA ACAAACAGTACGAGCTGCAA 45.0 45.0 58.0 58.1 985 bp 0.5 59.4
ndhG-ndhI_p3 CATGTATCGGTCCAGGCAAA AACAAACAGTACGAGCTGCA 50.0 45.0 58.0 58.1 840 bp 0.5 59.3
ndhG-ndhI_p4 CATGTATCGGTCCAGGCAAA ACAAACAGTACGAGCTGCAA 50.0 45.0 58.0 58.1 839 bp 0.5 59.3
ndhG-ndhI_p5 CAAGAACCAGTCCCAACGAA ACAAACAGTACGAGCTGCAA 50.0 45.0 58.0 58.1 944 bp 0.5 59.3