Hotspot

rpl32-trnL-TAG

SSC · 115565-116725 · 1161 bp

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Pi 0.428
MarkerSeek score 79.90
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
1161
variable_sites
394
pi
0.428
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 GGCAGCGTTGAAAGCTTTTT GGAGAGCGGGGACTAATAGT 45.0 55.0 58.4 58.0 1822 bp 0.5 56.6
rpl32-trnL-TAG_p2 GGCAGCGTTGAAAGCTTTTT TGGAGAGCGGGGACTAATAG 45.0 55.0 58.4 58.0 1823 bp 0.5 56.5
rpl32-trnL-TAG_p3 GGCAGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.4 58.0 1275 bp 0.5 56.5
rpl32-trnL-TAG_p4 GGCAGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.4 58.0 1276 bp 0.5 56.5
rpl32-trnL-TAG_p5 GGCAGCGTTGAAAGCTTTTT CGGGGACTAATAGTGGGGAT 45.0 55.0 58.4 58.0 1816 bp 0.5 56.4