Hotspot

ndhF-rpl32

SSC · 114749-115402 · 654 bp

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Pi 0.388
MarkerSeek score 78.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
654
variable_sites
192
pi
0.388
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.76
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAAGTAGAAGTCCCGCTCCT CGCCCTTTGCTTTTCCAAAT 50.0 45.0 58.1 57.8 813 bp 0.5 58.2
ndhF-rpl32_p2 GAAGGTTTTTGGTTGCCGTC CGCCCTTTGCTTTTCCAAAT 50.0 45.0 58.2 57.8 837 bp 0.5 57.4
ndhF-rpl32_p3 GTCGAAGGTTTTTGGTTGCC CGCCCTTTGCTTTTCCAAAT 50.0 45.0 58.2 57.8 840 bp 0.5 57.4
ndhF-rpl32_p4 GTGGATCGATCAAGTAGCCC CGCCCTTTGCTTTTCCAAAT 55.0 45.0 57.8 57.8 1002 bp 0.5 57.4
ndhF-rpl32_p5 TGGATCGATCAAGTAGCCCA CGCCCTTTGCTTTTCCAAAT 50.0 45.0 58.2 57.8 1001 bp 0.5 57.0