Hotspot

clpP

LSC · 75675-77924 · 2250 bp

Back to Ludwigia

Pi 0.0355

MarkerSeek score 72.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2250
variable_sites: 171
pi: 0.0355
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	ATACGCAATGGGGGTTGATC	50.0	50.0	58.0	58.0	2534-2725 bp	1.0	90.2
clpP_p2	ATTTTGTCCCAGCGAGGATC	ATACGCAATGGGGGTTGATC	50.0	50.0	58.0	58.0	2553-2744 bp	1.0	90.0
clpP_p3	CTGAACGAGTCGCACATACA	ACTATGCTATGGACCTCCCC	50.0	55.0	58.0	58.0	2680-2871 bp	1.0	89.8
clpP_p4	CTGAACGAGTCGCACATACA	GAACAGAAAGCAAACGACCG	50.0	50.0	58.0	58.0	2805-2996 bp	1.0	89.6
clpP_p5	ATTTTGTCCCAGCGAGGATC	GAACAGAAAGCAAACGACCG	50.0	50.0	58.0	58.0	2824-2995 bp	0.8	79.2