Hotspot

ccsA-ndhD

SSC · 120814-121203 · 390 bp

Back to Ludwigia

Pi 0.0282

MarkerSeek score 65.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 390
variable_sites: 22
pi: 0.0282
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGGTTACATAGTTATGGTTCA	TCAGTTGATAAGGTGGAAGC	36.4	45.0	54.2	55.1	469-479 bp	1.0	44.7
ccsA-ndhD_p2	AGGGTTACATAGTTATGGTTCA	ATCCGGATTTCGTTCTTTCA	36.4	40.0	54.2	55.1	492-502 bp	1.0	44.5
ccsA-ndhD_p3	AGGGTTACATAGTTATGGTTCA	TCCGGATTTCGTTCTTTCAT	36.4	40.0	54.2	55.1	491-501 bp	1.0	44.5
ccsA-ndhD_p4	AGGGTTACATAGTTATGGTTCA	TCCGGATTTCGTTCTTTCATT	36.4	38.1	54.2	55.9	491-501 bp	1.0	44.5
ccsA-ndhD_p5	AGGGTTACATAGTTATGGTTCA	ATCCGGATTTCGTTCTTTCAT	36.4	38.1	54.2	55.7	492-502 bp	1.0	44.5