Hotspot

rps8-rpl14

LSC · 86470-86715 · 246 bp

Back to Ludwigia

Pi 0.0275

MarkerSeek score 64.20

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 15
pi: 0.0275
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	AGTTTCATTAGCACCTGAGGT	50.0	42.9	57.7	56.9	274-333 bp	1.0	69.2
rps8-rpl14_p2	AGCAATAGTGTCCCTACCCA	GTTTCATTAGCACCTGAGGT	50.0	45.0	57.7	55.4	273-332 bp	1.0	64.7
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	AGTTTCATTAGCACCTGAGG	50.0	45.0	57.7	55.0	274-333 bp	1.0	62.2
rps8-rpl14_p4	AGCAATAGTGTCCCTACCCAT	AGTTTCATTAGCACCTGAGGT	47.6	42.9	58.2	56.9	274-333 bp	1.0	61.6
rps8-rpl14_p5	GCAATAGTGTCCCTACCCAT	AGTTTCATTAGCACCTGAGGT	50.0	42.9	56.7	56.9	273-332 bp	1.0	60.8