Hotspot

rps12-clpP

LSC · 75534-75674 · 141 bp

Back to Ludwigia

Pi 0.109

MarkerSeek score 72.60

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 30
pi: 0.109
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTGAACGAGTCGCACATACA	CCGAAGATCTGGAGAGGGAT	50.0	55.0	58.0	58.0	350-478 bp	1.0	87.8
rps12-clpP_p2	ACTCTCTATACACCGGAGCC	TGGGCGAATTTGTCTTGGAA	55.0	45.0	58.0	58.0	914-919 bp	0.8	76.1
rps12-clpP_p3	ACTCTCTATACACCGGAGCC	CCGAAGATCTGGAGAGGGAT	55.0	55.0	58.0	58.0	815-820 bp	0.8	75.7
rps12-clpP_p4	CTGAACGAGTCGCACATACA	ATCCGAAGATCTGGAGAGGG	50.0	55.0	58.0	58.0	352-353 bp	0.6	67.2
rps12-clpP_p5	ACTCTCTATACACCGGAGCC	ATCCGAAGATCTGGAGAGGG	55.0	55.0	58.0	58.0	817-822 bp	0.6	66.0