Hotspot

rpoB-trnC-GCA

LSC · 28341-29634 · 1294 bp

Back to Ludwigia

Pi 0.0147

MarkerSeek score 67.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1294
variable_sites: 44
pi: 0.0147
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCATTTCCATCCTCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1323-1376 bp	1.0	83.5
rpoB-trnC-GCA_p2	CCATTTCCATCCTCGAGCAT	TTGTTAATGAGGCGACACCC	50.0	50.0	57.7	58.2	1341-1394 bp	1.0	82.6
rpoB-trnC-GCA_p3	ACATTCCCCCATTTCCATCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1331-1384 bp	1.0	81.8
rpoB-trnC-GCA_p4	CCATTTCCATCCTCGAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1305-1358 bp	1.0	81.4
rpoB-trnC-GCA_p5	ACATTCCCCCATTTCCATCC	TTGTTAATGAGGCGACACCC	50.0	50.0	57.5	58.2	1349-1402 bp	1.0	80.9