Hotspot

trnK-UUU-rps16

LSC · 4443-5333 · 891 bp

Back to Ludwigia

Pi 0.0228

MarkerSeek score 65.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 891
variable_sites: 47
pi: 0.0228
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CAGTTCAGGATCAGTCGTGG	AATTGATGTGCGATCCCGAA	55.0	45.0	58.0	58.0	1225-1248 bp	1.0	84.5
trnK-UUU-rps16_p2	GTTCAGGATCAGTCGTGGTC	AATTGATGTGCGATCCCGAA	55.0	45.0	58.0	58.0	1223-1246 bp	1.0	84.5
trnK-UUU-rps16_p3	CAGTTCAGGATCAGTCGTGG	ATCGTTGCAATTGATGTGCG	55.0	45.0	58.0	58.1	1233-1256 bp	1.0	84.0
trnK-UUU-rps16_p4	CAGTTCAGGATCAGTCGTGG	CGTTGCAATTGATGTGCGAT	55.0	45.0	58.0	58.1	1231-1254 bp	1.0	84.0
trnK-UUU-rps16_p5	GTTCAGGATCAGTCGTGGTC	CGTTGCAATTGATGTGCGAT	55.0	45.0	58.0	58.1	1229-1252 bp	1.0	84.0