Hotspot

ycf1

SSC · 123622-129066 · 5445 bp

Back to Loxostigma

Pi 0.0237

MarkerSeek score 53.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5445
variable_sites: 128
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACTTGTAGAGGTCAGGGGA	GCAGAATACCGTCACCCATT	55.0	50.0	57.8	58.0	565-601 bp	1.0	84.7
ycf1_p2	AGACTTGTAGAGGTCAGGGG	GCAGAATACCGTCACCCATT	55.0	50.0	57.8	58.0	566-602 bp	1.0	84.7
ycf1_p3	GACTTGTAGAGGTCAGGGGA	TCCGAGCTCAGGTTATGGAA	55.0	50.0	57.8	58.1	1135-1171 bp	1.0	81.0
ycf1_p4	AGACTTGTAGAGGTCAGGGG	TCCGAGCTCAGGTTATGGAA	55.0	50.0	57.8	58.1	1136-1172 bp	1.0	80.9
ycf1_p5	GACTTGTAGAGGTCAGGGGA	CCGAGCTCAGGTTATGGAAG	55.0	55.0	57.8	57.8	1134-1170 bp	1.0	80.2