Hotspot

ndhF

SSC · 110438-112669 · 2232 bp

Back to Loxostigma

Pi 0.0238

MarkerSeek score 57.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 53
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	AACAAGGGAAAATACGGCGA	50.0	45.0	58.0	57.8	2575 bp	0.5	58.2
ndhF_p2	GCAGAATACCGTCACCCATT	ACAAGGGAAAATACGGCGAG	50.0	50.0	58.0	58.3	2574 bp	0.5	57.6
ndhF_p3	GCAGAATACCGTCACCCATT	CAAGGGAAAATACGGCGAGT	50.0	50.0	58.0	58.3	2573 bp	0.5	57.6
ndhF_p4	GCAGAATACCGTCACCCATT	AAGGGAAAATACGGCGAGTT	50.0	45.0	58.0	57.5	2572 bp	0.5	55.8
ndhF_p5	GCAGAATACCGTCACCCATT	AGGGAAAATACGGCGAGTTT	50.0	45.0	58.0	57.5	2571 bp	0.5	55.8