Hotspot

ndhD-psaC

SSC · 116992-117087 · 96 bp

Back to Loxostigma

Pi 0.0729

MarkerSeek score 70.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 96
variable_sites: 7
pi: 0.0729
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TCGTGGTAAAGACAAGATACA	ACTCGTAGTATGGGTCTAGCT	38.1	47.6	54.4	57.1	145 bp	1.0	43.9
ndhD-psaC_p2	TTCGTGGTAAAGACAAGATACA	ACTCGTAGTATGGGTCTAGCT	36.4	47.6	55.2	57.1	146 bp	1.0	43.8
ndhD-psaC_p3	TCGTGGTAAAGACAAGATACA	CGCAAGAAACAACTCGTAGT	38.1	45.0	54.4	56.4	156 bp	1.0	43.6
ndhD-psaC_p4	TTCGTGGTAAAGACAAGATACA	CGCAAGAAACAACTCGTAGT	36.4	45.0	55.2	56.4	157 bp	1.0	43.6
ndhD-psaC_p5	TCGTGGTAAAGACAAGATACAT	CGCAAGAAACAACTCGTAGT	36.4	45.0	54.9	56.4	156 bp	1.0	43.6