Hotspot

ndhF-rpl32

SSC · 112670-113430 · 761 bp

Back to Loxostigma

Pi 0.0414

MarkerSeek score 72.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 761
variable_sites: 31
pi: 0.0414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAAAGGTATGATCCATGCA	ACGATGCACAATACCCCTTC	42.9	50.0	56.8	58.0	887-918 bp	1.0	65.7
ndhF-rpl32_p2	AAGGAAAGGTATGATCCATGCA	ACGATGCACAATACCCCTTC	40.9	50.0	57.5	58.0	888-919 bp	1.0	63.4
ndhF-rpl32_p3	AGGAAAGGTATGATCCATGCA	AACGATGCACAATACCCCTT	42.9	45.0	56.8	57.5	888-919 bp	1.0	61.9
ndhF-rpl32_p4	AGGAAAGGTATGATCCATGCAT	ACGATGCACAATACCCCTTC	40.9	50.0	57.3	58.0	887-918 bp	1.0	61.7
ndhF-rpl32_p5	GGAAAGGTATGATCCATGCA	ACGATGCACAATACCCCTTC	45.0	50.0	55.2	58.0	886-917 bp	1.0	61.0