Hotspot

ycf4-cemA

LSC · 61667-62537 · 871 bp

Back to Loxostigma

Pi 0.0279
MarkerSeek score 67.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
871
variable_sites
24
pi
0.0279
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 GAACAAAAAGCCGCCGAATT GAGAGAGAAATCCAACAGGACA 45.0 45.5 58.2 57.5 995-1005 bp 1.0 60.7
ycf4-cemA_p2 GAACAAAAAGCCGCCGAATT TGAGAGAGAAATCCAACAGGAC 45.0 45.5 58.2 57.5 996-1006 bp 1.0 60.7
ycf4-cemA_p3 TTTGCGCGTACCAATTGAAG GAGAGAGAAATCCAACAGGACA 45.0 45.5 57.7 57.5 966-976 bp 1.0 59.9
ycf4-cemA_p4 TTTGCGCGTACCAATTGAAG TGAGAGAGAAATCCAACAGGAC 45.0 45.5 57.7 57.5 967-977 bp 1.0 59.9
ycf4-cemA_p5 GAACAAAAAGCCGCCGAATT TCCAACAGGACAAAAACACT 45.0 40.0 58.2 55.3 985-995 bp 1.0 59.5