Hotspot

trnG-UCC-trnR-UCU

LSC · 10255-10478 · 224 bp

Back to Loxostigma

Pi 0.0647

MarkerSeek score 78.60

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 13
pi: 0.0647
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGAAATCAATTGCGTCCAA	50.0	40.0	58.0	56.2	330-351 bp	1.0	72.6
trnG-UCC-trnR-UCU_p2	AGCCTTCCAAGCTAACGATG	GATGGAAATCAATTGCGTCCA	50.0	42.9	58.0	57.2	332-353 bp	1.0	72.5
trnG-UCC-trnR-UCU_p3	CCTAGCCTTCCAAGCTAACG	TGGAAATCAATTGCGTCCAA	55.0	40.0	58.1	56.2	333-354 bp	1.0	72.4
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	GATGGAAATCAATTGCGTCCA	55.0	42.9	58.1	57.2	335-356 bp	1.0	72.3
trnG-UCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	ATGGAAATCAATTGCGTCCA	50.0	40.0	58.0	56.0	331-352 bp	1.0	70.7