Hotspot

trnK-UUU-rps16

LSC · 4264-5082 · 819 bp

Back to Loxostigma

Pi 0.0246
MarkerSeek score 69.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
819
variable_sites
20
pi
0.0246
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GTCGGGGTCTTCCAAACTTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1005-1010 bp 1.0 80.4
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 923-928 bp 1.0 79.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 922-927 bp 1.0 79.4
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 937-942 bp 1.0 78.6
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 932-937 bp 1.0 78.4