Hotspot

trnH-GUG-psbA

LSC · 75-397 · 323 bp

Back to Loxostigma

Pi 0.109
MarkerSeek score 74.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
323
variable_sites
35
pi
0.109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCAGTGCTATGCATGGTTCC 55.0 50.0 58.1 58.0 772-792 bp 1.0 82.7
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CAGTGCTATGCATGGTTCCT 55.0 50.0 58.1 58.0 771-791 bp 1.0 82.7
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 786-806 bp 1.0 82.6
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 785-805 bp 1.0 82.6
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 787-807 bp 1.0 82.6