Hotspot

ndhE-ndhG

SSC · 121850-122090 · 241 bp

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Pi 0.0207
MarkerSeek score 64.40
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
241
variable_sites
5
pi
0.0207
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 AGTTCAAGACACATCAGGGC TTGATAGGTGCAATTGCCGT 50.0 45.0 57.8 58.2 381 bp 1.0 78.2
ndhE-ndhG_p2 TTTCGGCTCGTGATCAATCC TTGATAGGTGCAATTGCCGT 50.0 45.0 58.3 58.2 351 bp 1.0 77.0
ndhE-ndhG_p3 CGAACCATATTTCGGCTCGT TTGATAGGTGCAATTGCCGT 50.0 45.0 58.4 58.2 360 bp 1.0 76.3
ndhE-ndhG_p4 CGGCTCGTGATCAATCCATA TTGATAGGTGCAATTGCCGT 50.0 45.0 57.6 58.2 348 bp 1.0 76.3
ndhE-ndhG_p5 CAGGGCCCGAACCATATTTC TTGATAGGTGCAATTGCCGT 55.0 45.0 58.7 58.2 367 bp 1.0 74.3