Hotspot

ccsA-ndhD

SSC · 119058-119397 · 340 bp

Pi 0.0446

MarkerSeek score 67.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 340
variable_sites: 14
pi: 0.0446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGGCATATTAGTACCCGCTC	TCTGGACCACGGGAGTTATT	55.0	50.0	58.0	58.1	1041-1064 bp	1.0	83.2
ccsA-ndhD_p2	CGGCATATTAGTACCCGCTC	TTCTGGACCACGGGAGTTAT	55.0	50.0	58.0	58.1	1042-1065 bp	1.0	83.2
ccsA-ndhD_p3	CGGCATATTAGTACCCGCTC	TTATCGATGGCTTCCCTTGC	55.0	50.0	58.0	58.0	1269-1292 bp	1.0	82.8
ccsA-ndhD_p4	CGGCATATTAGTACCCGCTC	ATGGGTTTATTAGCGCTGCA	55.0	45.0	58.0	57.9	1397-1420 bp	1.0	82.5
ccsA-ndhD_p5	CGGCATATTAGTACCCGCTC	TCGCTAACCGATACAGGACT	55.0	50.0	58.0	58.0	1446-1469 bp	1.0	82.4