Hotspot

psbT-psbN

LSC · 77192-77251 · 60 bp

Back to Lovoa

Pi 0.0333
MarkerSeek score 71.70
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
60
variable_sites
2
pi
0.0333
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TCGAGAACCGCCTAAAGTTC GAGATCCGTTCGAGGAACAC 50.0 55.0 57.6 58.1 129 bp 1.0 77.7
psbT-psbN_p2 TCGAGAACCGCCTAAAGTTC AGAGATCCGTTCGAGGAACA 50.0 50.0 57.6 58.2 130 bp 1.0 77.0
psbT-psbN_p3 GAGAACCGCCTAAAGTTCCA GAGATCCGTTCGAGGAACAC 50.0 55.0 57.5 58.1 127 bp 1.0 76.8
psbT-psbN_p4 GAGAACCGCCTAAAGTTCCA AGAGATCCGTTCGAGGAACA 50.0 50.0 57.5 58.2 128 bp 1.0 76.2
psbT-psbN_p5 CGAGAACCGCCTAAAGTTCC GAGATCCGTTCGAGGAACAC 55.0 55.0 58.6 58.1 128 bp 1.0 75.4