Hotspot

rpl20-rps12

LSC · 71798-72560 · 763 bp

Back to Lovoa

Pi 0.00656
MarkerSeek score 62.60
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
763
variable_sites
5
pi
0.00656
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 AAGTCTTGAATGAGCCCCAC CCGAGGAACATGTACAAGGG 50.0 55.0 57.8 58.0 880-881 bp 1.0 78.7
rpl20-rps12_p2 AAGTCTTGAATGAGCCCCAC AACCCAAGACAGCCAATCAG 50.0 50.0 57.8 58.1 942-943 bp 1.0 78.2
rpl20-rps12_p3 TTTGTTCTACGTCTCCGAGC CCGAGGAACATGTACAAGGG 50.0 55.0 57.7 58.0 834-835 bp 1.0 77.6
rpl20-rps12_p4 AAGTCTTGAATGAGCCCCAC AGAAACGTCACGAAATCCCC 50.0 50.0 57.8 58.2 924-925 bp 1.0 77.3
rpl20-rps12_p5 AAGTCTTGAATGAGCCCCAC CCCAAGACAGCCAATCAGAA 50.0 50.0 57.8 57.8 940-941 bp 1.0 77.2