Hotspot

trnP-psaJ

LSC · 69535-69885 · 351 bp

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Pi 0.0456
MarkerSeek score 76.20
Primer available yes
Label trnP-psaJ

Feature Metadata

label_name
trnP-psaJ
feature_type
igs
region
LSC
length_bp
351
variable_sites
16
pi
0.0456
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-psaJ_p1 ACCCGTGACATTTTGTACCC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 441-453 bp 1.0 81.8
trnP-psaJ_p2 CCCGTGACATTTTGTACCCA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 440-452 bp 1.0 81.8
trnP-psaJ_p3 ACAGGATTTGAACCCGTGAC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 452-464 bp 1.0 81.6
trnP-psaJ_p4 CAGGATTTGAACCCGTGACA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 451-463 bp 1.0 81.6
trnP-psaJ_p5 TGACAGGATTTGAACCCGTG GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 454-466 bp 1.0 81.6