Hotspot

ndhC-trnV

LSC · 53045-53952 · 908 bp

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Pi 0.021
MarkerSeek score 58.10
Primer available yes
Label ndhC-trnV

Feature Metadata

label_name
ndhC-trnV
feature_type
igs
region
LSC
length_bp
908
variable_sites
19
pi
0.021
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV_p1 TCATATTTGTAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 27.3 50.0 52.0 57.8 959-964 bp 1.0 42.0
ndhC-trnV_p2 TCATATTTGTAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.7 57.8 959-964 bp 1.0 42.0
ndhC-trnV_p3 TCATATTTGTAAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 27.3 55.0 52.0 57.5 958-963 bp 1.0 42.0
ndhC-trnV_p4 TCATATTTGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 27.3 55.0 52.0 58.7 947-952 bp 1.0 42.0
ndhC-trnV_p5 TCATATTTGTAAAGCAGAAACA AAGGTCTACGGTTCGAGTCC 27.3 55.0 52.0 58.8 957-962 bp 1.0 42.0