Hotspot

trnF-ndhJ

LSC · 50939-51365 · 427 bp

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Pi 0.0164
MarkerSeek score 61.90
Primer available yes
Label trnF-ndhJ

Feature Metadata

label_name
trnF-ndhJ
feature_type
igs
region
LSC
length_bp
427
variable_sites
7
pi
0.0164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 569-575 bp 1.0 80.4
trnF-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 533-539 bp 1.0 80.1
trnF-ndhJ_p3 ATGGTGTGTTGGGAATGGTC AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 589-595 bp 1.0 79.8
trnF-ndhJ_p4 GATGGTGTGTTGGGAATGGT AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 590-596 bp 1.0 79.8
trnF-ndhJ_p5 CTGGTAGAGCAGAGGACTGA AGTTGGATAGGATGGCCCTT 55.0 50.0 57.9 58.1 557-563 bp 1.0 79.4