Hotspot

rps4-trnT

LSC · 48457-48818 · 362 bp

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Pi 0.0138
MarkerSeek score 61.70
Primer available yes
Label rps4-trnT

Feature Metadata

label_name
rps4-trnT
feature_type
igs
region
LSC
length_bp
362
variable_sites
5
pi
0.0138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT_p1 TAAGATCGCTTCCGGCTCTA TTTAAGCCCGCTTAGCTCAG 50.0 50.0 58.0 58.0 537-539 bp 1.0 80.4
rps4-trnT_p2 TTTCTAAGATCGCTTCCGGC TTTAAGCCCGCTTAGCTCAG 50.0 50.0 58.1 58.0 541-543 bp 1.0 80.0
rps4-trnT_p3 CTCGTAAGTCCCGGTAAAGC TTTAAGCCCGCTTAGCTCAG 55.0 50.0 58.1 58.0 508-510 bp 1.0 79.9
rps4-trnT_p4 TAAGATCGCTTCCGGCTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 522-524 bp 1.0 79.5
rps4-trnT_p5 TTTCTAAGATCGCTTCCGGC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 526-528 bp 1.0 79.2