Hotspot

rps16-trnQ

LSC · 6408-7767 · 1360 bp

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Pi 0.0148
MarkerSeek score 66.30
Primer available yes
Label rps16-trnQ

Feature Metadata

label_name
rps16-trnQ
feature_type
igs
region
LSC
length_bp
1360
variable_sites
20
pi
0.0148
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_p1 TCCATAGCGGATCATGTCCT GAAATTGAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1504-1513 bp 1.0 81.3
rps16-trnQ_p2 GCGGATCATGTCCTTCAAGT GAAATTGAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1498-1507 bp 1.0 81.2
rps16-trnQ_p3 TCCATAGCGGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1446-1455 bp 1.0 80.9
rps16-trnQ_p4 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1440-1449 bp 1.0 80.8
rps16-trnQ_p5 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1482-1491 bp 1.0 80.4