Hotspot

trnK-rps16

LSC · 4436-5296 · 861 bp

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Pi 0.0175
MarkerSeek score 59.80
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
861
variable_sites
15
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 AGCCGTACTTAAAAGCCGAG ATTTTCTTGAGAAGGGCGCT 50.0 45.0 58.0 57.8 1006-1010 bp 1.0 80.0
trnK-rps16_p2 AAAGCCGAGTACTCTACCGT ATTTTCTTGAGAAGGGCGCT 50.0 45.0 58.2 57.8 995-999 bp 1.0 78.7
trnK-rps16_p3 AAGCCGAGTACTCTACCGTT ATTTTCTTGAGAAGGGCGCT 50.0 45.0 58.2 57.8 994-998 bp 1.0 78.7
trnK-rps16_p4 AGCCGTACTTAAAAGCCGAG CGCTCAACCTACAGGAATCG 50.0 55.0 58.0 58.4 990-994 bp 1.0 78.1
trnK-rps16_p5 AGCCGTACTTAAAAGCCGAG TCAAAGAAGGCAGGGGTTTT 50.0 45.0 58.0 57.5 959-963 bp 1.0 77.9