Hotspot

ndhF-rpl32

SSC · 110010-110792 · 783 bp

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Pi 0.0334
MarkerSeek score 77.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
783
variable_sites
88
pi
0.0334
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGTGGGGTGAAGGGTATGAT AAACGCTTTCAATGCTGTCC 50.0 45.0 57.7 57.6 896-957 bp 1.0 86.0
ndhF-rpl32_p2 AGTGGGGTGAAGGGTATGAT TGCTGTCCAATATCCCCTCT 50.0 50.0 57.7 57.8 884-945 bp 0.846154 80.1
ndhF-rpl32_p3 GGGGTGAAGGGTATGATCCA TGCTGTCCAATATCCCCTCT 55.0 50.0 58.5 57.8 881-942 bp 0.846154 78.6
ndhF-rpl32_p4 TGGGGTGAAGGGTATGATCC TGCTGTCCAATATCCCCTCT 55.0 50.0 58.5 57.8 882-943 bp 0.846154 78.6
ndhF-rpl32_p5 GTGGGGTGAAGGGTATGATC TGCTGTCCAATATCCCCTCT 55.0 50.0 57.4 57.8 883-944 bp 0.846154 77.3