Hotspot

rpl36-rps8

LSC · 77963-78320 · 358 bp

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Pi 0.0349
MarkerSeek score 72.80
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
358
variable_sites
35
pi
0.0349
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 GCCTTCGGATCAATCGACAT TCGGCGGAGAAGTTTTATGT 50.0 45.0 58.1 57.2 418-528 bp 1.0 83.0
rpl36-rps8_p2 ACTATAATCCGACCTCGCCT TCGGCGGAGAAGTTTTATGT 50.0 45.0 57.7 57.2 434-544 bp 1.0 81.7
rpl36-rps8_p3 GCCTTCGGATCAATCGACAT TCGGCGGAGAAGTTTTATGTT 50.0 42.9 58.1 58.0 418-528 bp 1.0 80.6
rpl36-rps8_p4 TCTTACGAACAGACGCTCCT TCGGCGGAGAAGTTTTATGT 50.0 45.0 58.5 57.2 388-498 bp 1.0 80.5
rpl36-rps8_p5 ATCTTACGAACAGACGCTCC TCGGCGGAGAAGTTTTATGT 50.0 45.0 57.4 57.2 389-499 bp 1.0 79.7