Hotspot

rps11-rpl36

LSC · 77504-77848 · 345 bp

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Pi 0.046
MarkerSeek score 75.60
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
345
variable_sites
43
pi
0.046
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 CGAGAACCACTACGTGCATT CCAAGACATAAACAAAGACAGGG 50.0 43.5 58.3 57.6 416-450 bp 1.0 62.7
rps11-rpl36_p2 TTACGAGAACCACTACGTGC CCAAGACATAAACAAAGACAGGG 50.0 43.5 57.7 57.6 419-453 bp 1.0 62.3
rps11-rpl36_p3 TGCTTACGAGAACCACTACG CCAAGACATAAACAAAGACAGGG 50.0 43.5 57.4 57.6 422-456 bp 1.0 60.0
rps11-rpl36_p4 GCTTACGAGAACCACTACGT CCAAGACATAAACAAAGACAGGG 50.0 43.5 57.4 57.6 421-455 bp 1.0 60.0
rps11-rpl36_p5 TACGAGAACCACTACGTGCA CCAAGACATAAACAAAGACAGGG 50.0 43.5 58.8 57.6 418-452 bp 1.0 58.9