Hotspot

rpl20-rps12

LSC · 66770-67613 · 844 bp

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Pi 0.0207
MarkerSeek score 71.80
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
844
variable_sites
57
pi
0.0207
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CCTCCGTTTTCGCGCTATAT CGCCGAGGAACTTGTACTAG 50.0 55.0 58.2 57.8 885-913 bp 1.0 85.7
rpl20-rps12_p2 CCTCCGTTTTCGCGCTATAT AAGACAAGACAGCCAATCCG 50.0 50.0 58.2 58.2 945-973 bp 1.0 85.3
rpl20-rps12_p3 CCTCCGTTTTCGCGCTATAT GACAAGACAGCCAATCCGAA 50.0 50.0 58.2 58.2 943-971 bp 1.0 85.3
rpl20-rps12_p4 CCTCCGTTTTCGCGCTATAT ACAGCCAATCCGAAATGTCA 50.0 45.0 58.2 57.8 937-965 bp 1.0 85.3
rpl20-rps12_p5 AAGTCTTGAATGAGCTCCGC AAGACAAGACAGCCAATCCG 50.0 50.0 58.3 58.2 996-1024 bp 1.0 84.5