Hotspot

trnP-UGG-psaJ

LSC · 64206-64904 · 699 bp

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Pi 0.0371
MarkerSeek score 75.10
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
699
variable_sites
71
pi
0.0371
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.94
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG GGTGCCACAGAGAGATATGT 45.0 50.0 57.9 56.7 654-769 bp 1.0 80.7
trnP-UGG-psaJ_p2 CAAAACAAACGCGCTACCAA GGTGCCACAGAGAGATATGT 45.0 50.0 58.2 56.7 655-770 bp 1.0 80.2
trnP-UGG-psaJ_p3 TTGTACCCAAAACAAACGCG GGTGCCACAGAGAGATATGT 45.0 50.0 57.8 56.7 662-777 bp 1.0 80.0
trnP-UGG-psaJ_p4 AAAACAAACGCGCTACCAAG GGTGCCACAGAGAGATATGTT 45.0 47.6 57.9 57.4 654-769 bp 1.0 78.6
trnP-UGG-psaJ_p5 TACCCAAAACAAACGCGCTA GGTGCCACAGAGAGATATGT 45.0 50.0 58.4 56.7 659-774 bp 1.0 78.3