Hotspot

trnW-CCA-trnP-UGG

LSC · 63953-64131 · 179 bp

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Pi 0.0473
MarkerSeek score 74.10
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
179
variable_sites
24
pi
0.0473
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGTTCTACCGAACTGAACT GGTTCAAATCCTGTCATCCCT 50.0 47.6 57.9 57.6 225-240 bp 1.0 80.5
trnW-CCA-trnP-UGG_p2 ACCTACGACATCGGGTTTTG GGTTCAAATCCTGTCATCCCT 50.0 47.6 57.9 57.6 252-267 bp 1.0 79.8
trnW-CCA-trnP-UGG_p3 ACCGAACTGAACTAAGAGCG GGTTCAAATCCTGTCATCCCT 50.0 47.6 57.7 57.6 218-233 bp 1.0 78.2
trnW-CCA-trnP-UGG_p4 TACGACATCGGGTTTTGGAG GGTTCAAATCCTGTCATCCCT 50.0 47.6 57.6 57.6 249-264 bp 1.0 77.6
trnW-CCA-trnP-UGG_p5 CTACGACATCGGGTTTTGGA GGTTCAAATCCTGTCATCCCT 50.0 47.6 57.6 57.6 250-265 bp 1.0 77.5