Hotspot

petA-psbJ

LSC · 61463-62266 · 804 bp

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Pi 0.0242
MarkerSeek score 75.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
804
variable_sites
67
pi
0.0242
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTGTCCAAGGTCTCTTGTT TGGTTCGTATTCCGGATTGG 50.0 50.0 57.8 57.7 541-964 bp 1.0 86.3
petA-psbJ_p2 CGTTACGTGTCCAAGGTCTC TGGTTCGTATTCCGGATTGG 55.0 50.0 58.3 57.7 546-969 bp 1.0 85.8
petA-psbJ_p3 CGTGTCCAAGGTCTCTTGTT GGTTCGTATTCCGGATTGGG 50.0 55.0 57.8 58.4 540-963 bp 1.0 85.5
petA-psbJ_p4 GATCCGTTACGTGTCCAAGG TGGTTCGTATTCCGGATTGG 55.0 50.0 58.4 57.7 550-973 bp 1.0 85.3
petA-psbJ_p5 CGTTACGTGTCCAAGGTCTC GGTTCGTATTCCGGATTGGG 55.0 55.0 58.3 58.4 545-968 bp 1.0 85.0