Hotspot

rps16-accD

LSC · 56107-56646 · 540 bp

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Pi 0.0387
MarkerSeek score 76.70
Primer available yes
Label rps16-accD

Feature Metadata

label_name
rps16-accD
feature_type
igs
region
LSC
length_bp
540
variable_sites
69
pi
0.0387
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-accD_p1 GGTGCTCAACCTACAGGAAC ACCTGAACCACCATTTTTCCA 55.0 42.9 58.2 58.0 628-718 bp 0.153846 37.9
rps16-accD_p2 GTGCTCAACCTACAGGAACT ACCTGAACCACCATTTTTCCA 50.0 42.9 57.2 58.0 627-717 bp 0.153846 32.7
rps16-accD_p3 GGTGCTCAACCTACAGGAAC CCTGAACCACCATTTTTCCA 55.0 45.0 58.2 56.1 627-717 bp 0.153846 31.1
rps16-accD_p4 GGTGCTCAACCTACAGGAAC ACCTGAACCACCATTTTTCC 55.0 45.0 58.2 56.1 628-718 bp 0.153846 31.1
rps16-accD_p5 GGCAGGTTTCTTTATGGACCT ACCTGAACCACCATTTTTCCA 47.6 42.9 57.9 58.0 587-677 bp 0.153846 30.8