Hotspot

trnQ-UUG-rps16

LSC · 54480-54976 · 497 bp

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Pi 0.0428
MarkerSeek score 78.50
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
497
variable_sites
60
pi
0.0428
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.89
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAGGTTCGAATCCTTCCGTC GTCTTTCAAGTCGCACGTTG 55.0 50.0 58.1 58.0 514-577 bp 1.0 91.3
trnQ-UUG-rps16_p2 GTCGAATCAAAAATGGGGCG GTCTTTCAAGTCGCACGTTG 50.0 50.0 58.1 58.0 575-638 bp 1.0 91.2
trnQ-UUG-rps16_p3 GAATCAAAAATGGGGCGTGG GTCTTTCAAGTCGCACGTTG 50.0 50.0 58.0 58.0 572-635 bp 0.923077 88.8
trnQ-UUG-rps16_p4 GAATCAAAAATGGGGCGTGG ATCACGTCTTTCAAGTCGCA 50.0 45.0 58.0 57.9 577-640 bp 0.923077 87.7
trnQ-UUG-rps16_p5 GAATCAAAAATGGGGCGTGG GCACGTTGCTTTTTACCACA 50.0 45.0 58.0 57.8 560-623 bp 0.923077 87.3