Hotspot

trnG-UCC-trnS-GCU

LSC · 52034-52819 · 786 bp

Back to Lotus

Pi 0.035
MarkerSeek score 79.50
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
786
variable_sites
91
pi
0.035
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 GGGAGTCCATAGAACGAATCG CAATCCGACGCTTTAGTCCA 52.4 50.0 58.0 58.0 663-894 bp 1.0 83.8
trnG-UCC-trnS-GCU_p2 GGGAGTCCATAGAACGAATCG AATCCGACGCTTTAGTCCAC 52.4 50.0 58.0 58.0 662-893 bp 1.0 83.8
trnG-UCC-trnS-GCU_p3 GAGTCCATAGAACGAATCGCA CAATCCGACGCTTTAGTCCA 47.6 50.0 57.9 58.0 661-892 bp 1.0 83.3
trnG-UCC-trnS-GCU_p4 GAGTCCATAGAACGAATCGCA AATCCGACGCTTTAGTCCAC 47.6 50.0 57.9 58.0 660-891 bp 1.0 83.3
trnG-UCC-trnS-GCU_p5 GGGAGTCCATAGAACGAATCG ACGGAAAGAGAGGGATTCGA 52.4 50.0 58.0 58.2 716-947 bp 1.0 82.7