Hotspot

atpA-trnR-UCU

LSC · 50678-50972 · 295 bp

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Pi 0.0326
MarkerSeek score 65.90
Primer available yes
Label atpA-trnR-UCU

Feature Metadata

label_name
atpA-trnR-UCU
feature_type
igs
region
LSC
length_bp
295
variable_sites
32
pi
0.0326
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-trnR-UCU_p1 TCAGACCCGCGATTAATGTG AGCCTTCCAAGCTAACGATG 50.0 50.0 58.1 58.0 976-1071 bp 1.0 89.6
atpA-trnR-UCU_p2 TCAGACCCGCGATTAATGTG ACCCTTAGCCTTCCAAGCTA 50.0 50.0 58.1 58.0 982-1077 bp 1.0 89.5
atpA-trnR-UCU_p3 CGAAGCTTATCCGGGAGATG AGCCTTCCAAGCTAACGATG 55.0 50.0 57.9 58.0 1185-1280 bp 0.923077 85.6
atpA-trnR-UCU_p4 CGAAGCTTATCCGGGAGATG ACCCTTAGCCTTCCAAGCTA 55.0 50.0 57.9 58.0 1191-1286 bp 0.923077 85.5
atpA-trnR-UCU_p5 TGGAACGAGCTGCGAAATTA AGCCTTCCAAGCTAACGATG 45.0 50.0 57.9 58.0 1152-1236 bp 0.923077 85.5