Hotspot

atpH-atpF

LSC · 47235-47776 · 542 bp

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Pi 0.0314
MarkerSeek score 73.00
Primer available yes
Label atpH-atpF

Feature Metadata

label_name
atpH-atpF
feature_type
igs
region
LSC
length_bp
542
variable_sites
54
pi
0.0314
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpF_p1 TGGGCTGGTTGTAGCATTAG GGAAAGGATCGGTTATATTTTTCA 50.0 33.3 57.6 55.2 610-658 bp 1.0 50.9
atpH-atpF_p2 TTTATGGGCTGGTTGTAGCA GGAAAGGATCGGTTATATTTTTCA 45.0 33.3 57.1 55.2 614-662 bp 1.0 50.9
atpH-atpF_p3 GGCTGGTTGTAGCATTAGCA GGAAAGGATCGGTTATATTTTTCA 50.0 33.3 58.2 55.2 608-656 bp 0.923077 47.4
atpH-atpF_p4 GGCTGGTTGTAGCATTAGCA GGAAAGGATCGGTTATATTTTTCAT 50.0 32.0 58.2 55.7 608-656 bp 0.923077 47.4
atpH-atpF_p5 GCTGGTTGTAGCATTAGCAC GGAAAGGATCGGTTATATTTTTCA 50.0 33.3 57.2 55.2 607-655 bp 0.923077 47.4