Hotspot

trnC-GCA-rpoB

LSC · 33174-34087 · 914 bp

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Pi 0.0306
MarkerSeek score 71.90
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
914
variable_sites
95
pi
0.0306
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 CCGGATTTGAACTGGGGAAA CATTTGCAACCCCAAGCAT 50.0 47.4 58.1 57.1 795-1003 bp 1.0 75.8
trnC-GCA-rpoB_p2 AAAAGGATTTGCAGTCCCCC CATTTGCAACCCCAAGCAT 50.0 47.4 58.4 57.1 777-985 bp 1.0 73.6
trnC-GCA-rpoB_p3 GACACCCGGATTTGAACTGG CATTTGCAACCCCAAGCAT 55.0 47.4 58.8 57.1 800-1008 bp 1.0 69.8
trnC-GCA-rpoB_p4 CGACACCCGGATTTGAACTG CATTTGCAACCCCAAGCAT 55.0 47.4 59.2 57.1 801-1009 bp 1.0 66.8
trnC-GCA-rpoB_p5 CGACACCCGGATTTGAACT CATTTGCAACCCCAAGCAT 52.6 47.4 57.8 57.1 801-1009 bp 1.0 66.7