Hotspot

petN-trnC-GCA

LSC · 31962-33102 · 1141 bp

Back to Lotus

Pi 0.0315
MarkerSeek score 81.10
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
1141
variable_sites
110
pi
0.0315
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAGGCAAGACTTACTA TTTCCCCAGTTCAAATCCGG 50.0 50.0 58.0 58.1 1168-1220 bp 1.0 90.4
petN-trnC-GCA_p2 AGCCCAGGCAAGACTTACTA TTTATCGTTTTGGCGGCATG 50.0 45.0 58.0 57.7 1221-1273 bp 1.0 88.7
petN-trnC-GCA_p3 AGCCCAGGCAAGACTTACTA GGGGGACTGCAAATCCTTTT 50.0 50.0 58.0 58.4 1186-1238 bp 1.0 88.1
petN-trnC-GCA_p4 TCTTCCCCATACTACGAGGG TTTCCCCAGTTCAAATCCGG 55.0 50.0 57.6 58.1 1219-1271 bp 1.0 87.5
petN-trnC-GCA_p5 TACCATTAAAGCAGCCCAGG TTTCCCCAGTTCAAATCCGG 50.0 50.0 57.6 58.1 1180-1232 bp 1.0 87.2